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2025

Catalysis of Native Chemical Ligation and Expressed Protein Ligation by Alkylselenols.

​Sánchez-Campillo I, Gratacòs-Batlle E, Pérez-García S, Nguyen HS, Triola G, Mootz HD, Blanco-Canosa JB.

JACS Au. 2025 Dec 2;5(12):5948-5964. doi: 10.1021/jacsau.5c00793.

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Different chains for different gains: How acyl chain diversity shapes S-acylated protein function

Busquets Hernández C, Tsiotsia A, Pipitò L, Chamberlain LH, Greaves J, Triola G.

Prog Lipid Res. 2025 Nov;100:101354. doi: 10.1016/j.plipres.2025.101354. 

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2024

Kinetic and Mechanistic Studies of Native Chemical Ligation with Phenyl α-Selenoester Peptides.

Sánchez-Campillo I, Blanco-Canosa JB.

JACS Au. 2024 Oct 30;4(11):4374-4382. doi: 10.1021/jacsau.4c00705. 

Quantitative analysis of protein lipidation and acyl-CoAs reveals substrate preferences of the S-acylation machinery

​Busquets-Hernández C, Ribó S, Gratacós-Batlle E, Carbajo D, Tsiotsia A, Blanco-Canosa JB, Chamberlain LH, Triola G. . Chem Sci. 2024 Jul 9;15(32):12845-12855. doi: 10.1039/d4sc02235a. 

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2023

High-throughput discovery of novel small-molecule inhibitors of acid Ceramidase.

Aseeri M, Abad JL, Delgado A, Fabriàs G, Triola G, Casas J.

Enzyme Inhib Med Chem. 2023 Dec;38(1):343-348. doi: 10.1080/14756366.2022.2150183.

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2022

Dinuclear silver and gold bisNHC complexes as drug candidates for cancer therapy.

Quintana M, Rodriguez-Rius A, Vellé A, Vives S, Sanz Miguel PJ, Triola G.

Bioorg Med Chem. 2022 Aug 1;67:116814. doi: 10.1016/j.bmc.2022.116814. Epub 2022 May 10. 

A versatile o-aminoanilide linker for native chemical ligation

Sánchez-Campillo I, Miguel-Gracia J, Karamanis P, Blanco-Canosa JB.

Chem Sci. 2022 Aug 26;13(36):10904-10913. doi: 10.1039/d2sc04158h.

2021

Palmitoylation as a Key Regulator of Ras Localization and Function. 

Busquets-Hernández, C., Triola, G.  

Front Mol Biosci. 2021, Mar 17; 8:659861. doi: 10.3389/fmolb.2021.659861

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2020

​Native Chemical Ligation via N-Acylurea Thioester Surrogates Obtained by Fmoc Solid-Phase Peptide Synthesis.

​Palà-Pujadas J, Blanco-Canosa JB.

Methods Mol Biol. 2020;2133:141-161. doi: 10.1007/978-1-0716-0434-2_7.

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DAPLE protein inhibits nucleotide exchange on Gαs and Gαq via the same motif that activates Gαi.

Marivin A, Maziarz M, Zhao J, DiGiacomo V, Olmos Calvo I, Mann EA, Ear J, Blanco-Canosa JB, Ross EM, Ghosh P, Garcia-Marcos M. J

Biol Chem. 2020 Feb 21;295(8):2270-2284. doi: 10.1074/jbc.RA119.011648

2019

A hydroxylamine probe for profiling S-acylated fatty acids on proteins.

Schulte-Zweckel J , Dwivedi M , Brockmeyer A , Janning P , Winter R , Triola G .  

Chem Commun (Camb). 2019;55(75):11183-11186. doi:10.1039/c9cc05989j

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Identification of benzo[cd]indol-2(1H)-ones as novel Atg4B inhibitors via a structure-based virtual screening and a novel AlphaScreen assay. 

Quintana M, Bilbao A, Comas-Barceló J, Bujons J, Triola G. 

Eur J Med Chem. 2019;178:648-666. doi:10.1016/j.ejmech.2019.05.086

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Activity-Based Imaging of Acid Ceramidase in Living Cells. 

Ordóñez YF, Abad JL, Aseeri M, Casas J, Garcia V, Casasampere M, Schuchman EH, Levade T, Delgado A, Triola G, Fabrias G.

J Am Chem Soc. 2019;141(19):7736-7742. doi:10.1021/jacs.8b11687

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Cancer Immunotherapy of TLR4 Agonist-Antigen Constructs Enhanced with Pathogen-Mimicking Magnetite Nanoparticles and Checkpoint Blockade of PD-L1. 

Traini G, Ruiz-de-Angulo A, Blanco-Canosa JB, Zamacola Bascarán K, Molinaro A, Silipo A, Escors D, Mareque-Rivas JC.

Small. 2019;15(4):e1803993. doi:10.1002/smll.201803993

2018

Peptide Ligations by Using Aryloxycarbonyl-o-methylaminoanilides: Chemical Synthesis of Palmitoylated Sonic Hedgehog. 

Palà-Pujadas J, Albericio F, Blanco-Canosa JB.

Angew Chem Int Ed Engl. 2018;57(49):16120-16125. doi:10.1002/anie.201810712

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A biochemical and genetic discovery pipeline identifies PLCδ4b as a nonreceptor activator of heterotrimeric G-proteins.

Maziarz M, Broselid S, DiGiacomo V, Park JC, Luebbers A, Garcia-Navarrete L, Blanco-Canosa JB, Baillie GS, Garcia-Marcos M. 

J Biol Chem. 2018;293(44):16964-16983. doi:10.1074/jbc.RA118.003580

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One-Pot Peptide Ligation-Oxidative Cyclization Protocol for the Preparation of Short-/Medium-Size Disulfide Cyclopeptides.

Spengler J, Blanco-Canosa JB, Forni L, Albericio F.  

Org Lett. 2018;20(14):4306-4309. doi:10.1021/acs.orglett.8b01741

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2017

Specific inhibition of GPCR-independent G protein signaling by a rationally engineered protein.

Leyme A, Marivin A, Maziarz M, DiGiacomo V, Papakonstantinou MP, Patel PP, Blanco-Canosa JB, Walawalkar IA, Rodriguez-Davila G, Dominguez I, Garcia-Marcos M.  

Proc Natl Acad Sci U S A. 2017;114(48):E10319-E10328. doi:10.1073/pnas.1707992114

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The Gαi-GIV binding interface is a druggable protein-protein interaction. 

DiGiacomo V, de Opakua AI, Papakonstantinou MP, Nguyen LT, Merino N, Blanco-Canosa JB, Blanco FJ, Garcia-Marcos M.

Sci Rep. 2017;7(1):8575. doi:10.1038/s41598-017-08829-7

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Molecular mechanism of Gαi activation by non-GPCR proteins with a Gα-Binding and Activating motif.

de Opakua AI, Parag-Sharma K, DiGiacomo V, Merino N, Leyme A, Marivin A, Villate M, Nguyen LT, de la Cruz-Morcillo MA, Blanco-Canosa JB, Ramachandran S, Baillie GS, Cerione RA, Blanco FJ, Garcia-Marcos M. 

Nat Commun. 2017;8:15163.  doi:10.1038/ncomms15163doi:10.1038/ncomms15163

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Concurrent Modulation of Quantum Dot Photoluminescence Using a Combination of Charge Transfer and Förster Resonance Energy Transfer: Competitive Quenching and Multiplexed Biosensing Modality

Algar WR, Khachatrian A, Melinger JS, Huston AL, Stewart MH, Susumu K, Blanco-Canosa JB, Oh E, Dawson PE, Medintz IL. 

J Am Chem Soc. 2017;139(1):363-372. doi:10.1021/jacs.6b11042

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2016

GIV/Girdin activates Gαi and inhibits Gαs via the same motif. 

Gupta V, Bhandari D, Leyme A, Aznar N, Midde KK, Lo IC, Ear J, Niesman I, López-Sánchez I,Blanco-Canosa JB, von Zastrow M, Garcia-Marcos M, Farquhar MG, Ghosh P.

Proc Natl Acad Sci U S A. 2016;113(39):E5721-E5730. doi:10.1073/pnas.1609502113

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High Affinity Immobilization of Proteins Using the CrAsH/TC Tag. 

Schulte-Zweckel J, Rosi F, Sreenu D, Schröder H, Niemeyer CM, Triola G.

Molecules. 2016;21(6):750. doi:10.3390/molecules21060750

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Guidelines for the use and interpretation of assays for monitoring autophagy

Klionsky DJ, Abdelmohsen K, Abe A, Abedin MJ, Abeliovich H, Acevedo Arozena A, Adachi H, Adams CM, Adams PD, Adeli K, Adhihetty PJ, Adler SG, Agam G, Agarwal R, Aghi MK, Agnello M, Agostinis P, Aguilar PV, Aguirre-Ghiso et al.

Autophagy. 2016;12(1):1-222. doi:10.1080/15548627.2015.1100356

2015

Specificity of Lipoprotein Chaperones for the Characteristic Lipidated Structural Motifs of their Cognate Lipoproteins. 

Mejuch T, van Hattum H, Triola G, Jaiswal M, Waldmann H.

Chembiochem. 2015;16(17):2460-2465. doi:10.1002/cbic.201500355

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Chemical Protein Synthesis Using a Second-Generation N-Acylurea Linker for the Preparation of Peptide-Thioester Precursors. 

Blanco-Canosa JB, Nardone B, Albericio F, Dawson PE.

J Am Chem Soc. 2015;137(22):7197-7209. doi:10.1021/jacs.5b03504

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2014

Site-specific, reversible and fluorescent immobilization of proteins on CrAsH-modified surfaces for microarray analytics. 

Schulte-Zweckel J, Rosi F, Sreenu D, Schröder H, Niemeyer CM, Triola G.

Chem Commun (Camb). 2014;50(84):12761-12764. doi:10.1039/c4cc04120h

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Structure guided design and kinetic analysis of highly potent benzimidazole inhibitors targeting the PDEδ prenyl binding site. 

Zimmermann G, Schultz-Fademrecht C, Küchler P, Murarka S, Ismail S, Triola G, Nussbaumer P, Wittinghofer A, Waldmann H.

J Med Chem. 2014;57(12):5435-5448. doi:10.1021/jm500632s

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Interaction of the human N-Ras protein with lipid raft model membranes of varying degrees of complexity. 

Vogel A, Nikolaus J, Weise K, Triola G, Waldmann H, Winter R, Herrmann A, Huster D.

Biol Chem. 2014;395(7-8):779-789. doi:10.1515/hsz-2013-0294

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Probing the Quenching of Quantum Dot Photoluminescence by Peptide-Labeled Ruthenium(II) Complexes. 

Scott AM, Algar WR, Stewart MH, Trammell SA, Blanco-Canosa JB, Dawson PE, Deschamps JR, Goswami R, Oh E, Huston AL, Medintz IL.

J Phys Chem C Nanomater Interfaces. 2014;118(17):9239-9250. doi:10.1021/jp501039w

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2013

Rotational and translational dynamics of ras proteins upon binding to model membrane systems. 

Werkmüller A, Triola G, Waldmann H, Winter R.

Chemphyschem. 2013;14(16):3698-3705. doi:10.1002/cphc.201300617

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Synthesis of lipidated peptides. 

Rosi F, Triola G.

Methods Mol Biol. 2013;1047:161-189. doi:10.1007/978-1-62703-544-6_12

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Synthesizing and modifying peptides for chemoselective ligation and assembly into quantum dot-peptide bioconjugates. 

Algar WR, Blanco-Canosa JB, Manthe RL, Susumu K, Stewart MH, Dawson PE, Medintz IL.

Methods Mol Biol. 2013;1025:47-73. doi:10.1007/978-1-62703-462-3_5

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Cytotoxicity of quantum dots used for in vitro cellular labeling: role of QD surface ligand, delivery modality, cell type, and direct comparison to organic fluorophores. 

Bradburne CE, Delehanty JB, Boeneman Gemmill K, Mei BC, Mattoussi H, Susumu K, Blanco-Canosa JB, Dawson PE, Medintz IL.

Bioconjug Chem. 2013;24(9):1570-1583. doi:10.1021/bc4001917

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Site-specific cellular delivery of quantum dots with chemoselectively-assembled modular peptides. 

Delehanty JB, Blanco-Canosa JB, Bradburne CE, Susumu K, Stewart MH, Prasuhn DE, Dawson PE, Medintz IL.

Chem Commun (Camb). 2013;49(72):7878-7880. doi:10.1039/c3cc42781a

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Imidazole-1-sulfonyl azide-based diazo-transfer reaction for the preparation of azido solid supports for solid-phase synthesis. 

Castro V, Blanco-Canosa JB, Rodriguez H, Albericio F.

ACS Comb Sci. 2013;15(7):331-334. 

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Semisynthetic lipidated LC3 protein mediates membrane fusion. 

Chembiochem. 2013;14(11):1296-1300. Yang A, Li Y, Pantoom S, Triola G, Wu YW.

doi:10.1002/cbic.201300344

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Gibbs energy determinants of lipoprotein insertion into lipid membranes: the case study of Ras proteins. 

Weise K, Huster D, Kapoor S, Triola G, Waldmann H, Winter R.

Faraday Discuss. 2013;161:549-589. doi:10.1039/c2fd20100c

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Selecting improved peptidyl motifs for cytosolic delivery of disparate protein and nanoparticle materials. 

Boeneman K, Delehanty JB, Blanco-Canosa JB, Susumu K, Stewart MH, Oh E, Huston AL, Dawson G, Ingale S, Walters R, Domowicz M, Deschamps JR, Algar WR, Dimaggio S, Manono J, Spillmann CM, Thompson D, Jennings TL, Dawson PE, Medintz IL.

ACS Nano. 2013;7(5):3778-3796. doi:10.1021/nn400702r

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Small molecule inhibition of the KRAS-PDEδ interaction impairs oncogenic KRAS signalling. 

Zimmermann G, Papke B, Ismail S, Vartak N, Chandra A, Hoffmann M, Hahn SA, Triola G, Wittinghofer A, Bastiaens PI, Waldmann H.

Nature. 2013;497(7451):638-642. doi:10.1038/nature12205

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2012

N-Ras forms dimers at POPC membranes. 

Güldenhaupt J, Rudack T, Bachler P, Mann D, Triola G, Waldmann H, Kötting C, Gerwert K.

Biophys J. 2012;103(7):1585-1593. doi:10.1016/j.bpj.2012.08.043

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Direct immobilization of oxyamine-modified proteins from cell lysates. 

Yi L, Chen YX, Lin PC, Schröder H, Niemeyer CM, Wu YW, Goody RS, Triola G, Waldmann H.

Chem Commun (Camb). 2012;48(88):10829-10831. doi:10.1039/c2cc35237k

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The role of G-domain orientation and nucleotide state on the Ras isoform-specific membrane interaction. 

Kapoor S, Weise K, Erlkamp M, Triola G, Waldmann H, Winter R.

Eur Biophys J. 2012;41(10):801-813. doi:10.1007/s00249-012-0841-5

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Dissociation of the K-Ras4B/PDEδ complex upon contact with lipid membranes: membrane delivery instead of extraction. 

Weise K, Kapoor S, Werkmüller A, Möbitz S, Zimmermann G, Triola G, Waldmann H, Winter R.

J Am Chem Soc. 2012;134(28):11503-11510. doi:10.1021/ja305518h

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Proteolytic activity at quantum dot-conjugates: kinetic analysis reveals enhanced enzyme activity and localized interfacial "hopping". 

Algar WR, Malonoski A, Deschamps JR, Blanco-Canosa JB, Susumu K, Stewart MH, Johnson BJ, Dawson PE, Medintz IL.

Nano Lett. 2012;12(7):3793-3802. doi:10.1021/nl301727k

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Complex Förster energy transfer interactions between semiconductor quantum dots and a redox-active osmium assembly. 

Stewart MH, Huston AL, Scott AM, Efros AL, Melinger JS, Gemmill KB, Trammell SA, Blanco-Canosa JB, Dawson PE, Medintz IL.

ACS Nano. 2012;6(6):5330-5347. doi:10.1021/nn301177h

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Chemical-biological exploration of the limits of the Ras de- and repalmitoylating machinery. 

Görmer K, Bürger M, Kruijtzer JA, Vetter I, Vartak N, Brunsveld L, Bastiaens PI, Liskamp RM, Triola G, Waldmann H.

Chembiochem. 2012;13(7):1017-1023. doi:10.1002/cbic.201200078

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Multimodal characterization of a linear DNA-based nanostructure. 

Buckhout-White S, Ancona M, Oh E, Deschamps JR, Stewart MH, Blanco-Canosa JB, Dawson PE, Goldman ER, Medintz IL.

ACS Nano. 2012;6(2):1026-1043. doi:10.1021/nn204680r

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Revealing conformational substates of lipidated N-Ras protein by pressure modulation. 

Kapoor S, Triola G, Vetter IR, Erlkamp M, Waldmann H, Winter R.

Proc Natl Acad Sci U S A. 2012;109(2):460-465. doi:10.1073/pnas.1110553109

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Chemical biology of lipidated proteins. 

Triola G, Waldmann H, Hedberg C.

ACS Chem Biol. 2012;7(1):87-99. doi:10.1021/cb200460u

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Quantum dots as simultaneous acceptors and donors in time-gated Förster resonance energy transfer relays: characterization and biosensing. 

Algar WR, Wegner D, Huston AL, Blanco-Canosa JB, Stewart MH, Armstrong A, Dawson PE, Hildebrandt N, Medintz IL.

J Am Chem Soc. 2012;134(3):1876-1891. 

2011

Arl2-GTP and Arl3-GTP regulate a GDI-like transport system for farnesylated cargo.

Ismail SA, Chen YX, Rusinova A, Chandra A, Bierbaum M, Gremer L, Triola G, Waldmann H, Bastiaens PI, Wittinghofer A.

Nat Chem Biol. 2011 Oct 16;7(12):942-9. doi: 10.1038/nchembio.686.

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Identification of acyl protein thioesterases 1 and 2 as the cellular targets of the Ras-signaling modulators palmostatin B and M.

Rusch M, Zimmermann TJ, Bürger M, Dekker FJ, Görmer K, Triola G, Brockmeyer A, Janning P, Böttcher T, Sieber SA, Vetter IR, Hedberg C, Waldmann H.

Angew Chem Int Ed Engl. 2011 Oct 10;50(42):9838-42. doi: 10.1002/anie.201102967. 

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Bioorthogonal chemistry for site-specific labeling and surface immobilization of proteins.

Chen YX, Triola G, Waldmann H.

Acc Chem Res. 2011 Sep 20;44(9):762-73. doi: 10.1021/ar200046h. 

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One-pot dual-labeling of a protein by two chemoselective reactions.

Yi L, Sun H, Itzen A, Triola G, Waldmann H, Goody RS, Wu YW.

Angew Chem Int Ed Engl. 2011 Aug 29;50(36):8287-90. doi: 10.1002/anie.201100840.

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Membrane-mediated induction and sorting of K-Ras microdomain signaling platforms.

Weise K, Kapoor S, Denter C, Nikolaus J, Opitz N, Koch S, Triola G, Herrmann A, Waldmann H, Winter R.

J Am Chem Soc. 2011 Feb 2;133(4):880-7. doi: 10.1021/ja107532q. 

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2010

A highly efficient strategy for modification of proteins at the C terminus.

Yi L, Sun H, Wu YW, Triola G, Waldmann H, Goody RS.

Angew Chem Int Ed Engl. 2010 Dec 3;49(49):9417-21. doi: 10.1002/anie.201003834.

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Synthesis of the Rheb and K-Ras4B GTPases.

Chen YX, Koch S, Uhlenbrock K, Weise K, Das D, Gremer L, Brunsveld L, Wittinghofer A, Winter R, Triola G, Waldmann H.

Angew Chem Int Ed Engl. 2010 Aug 16;49(35):6090-5. doi: 10.1002/anie.201001884. 

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Solid-phase synthesis of lipidated Ras peptides employing the Ellman sulfonamide linker.

Triola G, Gerauer M, Görmer K, Brunsveld L, Waldmann H.

Chemistry. 2010 Aug 16;16(31):9585-91. doi: 10.1002/chem.201001642.

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Influence of the lipid anchor motif of N-ras on the interaction with lipid membranes: a surface plasmon resonance study.

Gohlke A, Triola G, Waldmann H, Winter R.

Biophys J. 2010 May 19;98(10):2226-35. doi: 10.1016/j.bpj.2010.02.005. 

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Efficient microwave-assisted synthesis of unsymmetrical disulfides.

Görmer K, Waldmann H, Triola G.

J Org Chem. 2010 Mar 5;75(5):1811-3. doi: 10.1021/jo902695a. 

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Visualizing association of lipidated signaling proteins in heterogeneous membranes--partitioning into subdomains, lipid sorting, interfacial adsorption, and protein association.

Weise K, Triola G, Janosch S, Waldmann H, Winter R.

Biochim Biophys Acta. 2010 Jul;1798(7):1409-17. doi: 10.1016/j.bbamem.2009.12.006.

2009

cis- versus trans-ceramides: effects of the double bond on conformation and H-bonding interactions.

Phillips SC, Triola G, Fabrias G, Goñi FM, DuPré DB, Yappert MC.

J Phys Chem B. 2009 Nov 19;113(46):15249-55. doi: 10.1021/jp903000m.

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The lipid modifications of Ras that sense membrane environments and induce local enrichment.

Vogel A, Reuther G, Weise K, Triola G, Nikolaus J, Tan KT, Nowak C, Herrmann A, Waldmann H, Winter R, Huster D.

Angew Chem Int Ed Engl. 2009;48(46):8784-7. doi: 10.1002/anie.200903396.

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Influence of the lipidation motif on the partitioning and association of N-Ras in model membrane subdomains.

Weise K, Triola G, Brunsveld L, Waldmann H, Winter R.

J Am Chem Soc. 2009 Feb 4;131(4):1557-64. doi: 10.1021/ja808691r. 

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ATP competitive inhibitors of D-alanine-D-alanine ligase based on protein kinase inhibitor scaffolds.

Triola G, Wetzel S, Ellinger B, Koch MA, Hübel K, Rauh D, Waldmann H. Bioorg Med Chem. 2009 Feb 1;17(3):1079-87. doi: 10.1016/j.bmc.2008.02.046

2008

Racemization-free synthesis of S-alkylated cysteines via thiol-ene reaction.

Triola G, Brunsveld L, Waldmann H.

J Org Chem. 2008 May 2;73(9):3646-9. doi: 10.1021/jo800198s.

2006

Design, synthesis and activity as acid ceramidase inhibitors of 2-oxooctanoyl and N-oleoylethanolamine analogues.

Grijalvo S, Bedia C, Triola G, Casas J, Llebaria A, Teixidó J, Rabal O, Levade T, Delgado A, Fabriàs G.

Chem Phys Lipids. 2006 Oct;144(1):69-84. doi: 10.1016/j.chemphyslip.2006.07.001. 

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